sequencing adapters (New England Biolabs)

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New England Biolabs sequencing adapters
Sequencing Adapters, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 34027 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+sequences/T4+DNA+Ligase/10__3390_slash_arthropoda4040016-73-0-10
Average 99 stars, based on 34027 article reviews

sequencing adapters - by Bioz Stars, 2026-11

99/100 stars

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Generated:

Article Title: Evolution of insect innate immunity through domestication of bacterial toxins.
Article Snippet: .. We generated untagged cdtB from the pET-His6-TEV-cdtB [2B-T] plasmid (17) by standard mutagenesis. cdtB A (nt 61-840 of the cdtB aip56 A CDS), cdtB B (nt 61-840 of the cdtB aip56 B CDS), aip56 A (nt 841-1941 of the cdtB aip56 A CDS), and aip56 B (nt 841-1953 of the cdtB aip56 B CDS) were PCR amplified [using Q5 High-fidelity DNA polymerase (NEB)] from coding DNA sequences reverse transcribed from mRNA prepared from L. boulardi G486 infected wild-type D. ananassae larvae. .. To generate His6-tagged aip56-A and aip56-B or His6-TEV-tagged cdtB A and cdtB B, coding sequences were cloned into the pET-DUET1 (Merck-Millipore) or pDEST17 (Thermo Fisher Scientific) plasmids, respectively (without the predicted N-terminal signal peptides).

Article Title: Evolution of insect innate immunity through domestication of bacterial toxins
Article Snippet: .. We generated untagged cdtB from the pET-His6-TEV-cdtB [2B-T] plasmid ( ) by standard mutagenesis. cdtB A (nt 61-840 of the cdtB aip56 A CDS), cdtB B (nt 61-840 of the cdtB aip56 B CDS), aip56 A (nt 841-1941 of the cdtB aip56 A CDS), and aip56 B (nt 841-1953 of the cdtB aip56 B CDS) were PCR amplified [using Q5 High-fidelity DNA polymerase (NEB)] from coding DNA sequences reverse transcribed from mRNA prepared from L. boulardi G486 infected wild-type D. ananassae larvae. .. To generate His6-tagged aip56-A and aip56-B or His6-TEV-tagged cdtB A and cdtB B , coding sequences were cloned into the pET-DUET1 (Merck-Millipore) or pDEST17 (Thermo Fisher Scientific) plasmids, respectively (without the predicted N-terminal signal peptides).

Plasmid Preparation:

Mutagenesis:

Polymerase Chain Reaction:

Amplification:

Reverse Transcription:

Infection:

Ligation:

Article Title: Processing DNA Storage through Programmable Assembly in a Droplet-Based Fluidics System.
Article Snippet: .. For ligation, the 5′ ends of DNA sequences were phosphorylated with T4 polynucleotide kinase (New England BioLabs, Inc.) The phosphorylated DNA sequences were hybridized with splint DNA in the annealing process, during which the temperature was decreased from 95 to 30 °C by 1 °C min−1 in a SimpliAmp thermal cycler (ThermoFisher Scientific, Inc., Waltham, MA, USA). .. All DNA droplets were prepared to achieve a final concentration of 4 μm in 50 mm NaCl buffer (Samchun Pure Chemical Co., Seoul, Korea).

Article Title: Processing DNA Storage through Programmable Assembly in a Droplet‐Based Fluidics System
Article Snippet: .. For ligation, the 5′ ends of DNA sequences were phosphorylated with T4 polynucleotide kinase (New England BioLabs, Inc.) The phosphorylated DNA sequences were hybridized with splint DNA in the annealing process, during which the temperature was decreased from 95 to 30 °C by 1 °C min −1 in a SimpliAmp thermal cycler (ThermoFisher Scientific, Inc., Waltham, MA, USA). .. All DNA droplets were prepared to achieve a final concentration of 4 μ m in 50 m m NaCl buffer (Samchun Pure Chemical Co., Seoul, Korea).

Purification:

Article Title: A Mutated Nme1Cas9 Is a Functional Alternative RNase to Both LwaCas13a and RfxCas13d in the Yeast S. cerevisiae .
Article Snippet: .. Finally, the purified DNA sequences were ligated by T4 DNA ligase (NEB-M0202S) for 8 h at 16°C. ..

Article Title: A Mutated Nme1Cas9 Is a Functional Alternative RNase to Both LwaCas13a and RfxCas13d in the Yeast S. cerevisiae
Article Snippet: .. Finally, the purified DNA sequences were ligated by T4 DNA ligase (NEB-M0202S) for 8 h at 16°C. ..

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